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Restenosis is multifactorial in etiology Since the primary human-to-human cardiac transplant was that includes clean muscle cell proliferation buy ketoconazole cream visa antibiotics for uti in male, extracellular carried out efficiently by South African surgeon Dr matrix and native thrombosis buy 15gm ketoconazole cream with amex antibiotics for rabbit uti. However, widespread use of Christian Barnard in 1967, cardiac transplantation and drug-delivering stents has made it potential to beat prolonged assisted circulation is being carried out in lots of a number of lengthy-term problems of coronary stening. Most incessantly used is autologous graft of upper incidence of malignancy as a result of lengthy-term adminissaphenous vein which is reversed (as a result of valves within the vein) tration of immunosuppressive therapy. One of the primary and transplanted, or left inside mammary artery could also be issues in cardiac transplant centres is the provision of used being within the operative space of the guts. In a reversed saphenous vein graft, lengthy-term luminal into cardiac myocyte has generated interest in treatment of patency is 50% after 10 years. The regular grownup proper lung weighs 375 to 550 gm (average 450 gm) and is split by two fissures into three lobes�the higher, middle and decrease lobes. The weight of the normal grownup left lung is 325 to 450 gm (average four hundred gm) and has one fissure dividing it into two lobes�the higher and decrease lobes, while the center lobe is represented by the lingula. The airways of the lungs arise from the trachea by its division into proper and left major bronchi which proceed to divide and subdivide additional, ultimately terminating into the alveolar sacs (Fig. The proper major bronchus is extra vertical so that aspirated overseas material tends to pass down to the best lung rather than to the left. The trachea, major bronchi and their branchings possess cartilage, clean muscle and mucous glands of their partitions, while the bronchioles have clean muscle but lack cartilage as well as the mucous glands. Between the tracheal bifurcation and the smallest bronchi, about eight divisions take place. The bronchioles so fashioned additional undergo three to 4 divisions resulting in the terminal bronchioles which are lower than 2 mm in diameter. An acinus consists of 3 parts: glands and neuroendocrine cells which are bronchial counter1. Several (often three to 5 generations) respiratory bronchioles parts of the argentaffin cells of the alimentary tract originate from a terminal bronchiole. Each respiratory bronchiole divides into a number of alveolar of bronchi and its subdivisions as well as from alveoli. Each alveolar duct opens into many alveolar sacs (alveoli) ciliated epithelium but no mucus cells and hence, unlike the which are blind ends of the respiratory passages. In case of blockage of one side of circulation, the the alveolar partitions or alveolar septa are the websites of supply from the other can keep the vitality of pulmonary exchange between the blood and air and have the next parenchyma. The capillary endothelium lines the anastomotic capillaries intercommunicating lymphatics on the surface which drain within the alveolar partitions. The capillary endothelium and the, alveolar lining nodes obtain the lymph and drain into the thoracic duct. The bronchi and their subdivisions as much as consists of scanty amount of collagen, fibroblasts, nice elastic bronchioles are lined by pseudostratified columnar ciliated fibres, clean muscle cells, a couple of mast cells and mononuclear epithelial cells, also known as respiratory epithelium. The alveolar epithelium consists of two types of cells: kind I or decrease in quantity because the bronchioles are approached. Some of the essential circumstances from viewpoint of pathology are discussed beneath. A single giant cyst of this exhibits capillary endothelium, capillary basement membrane and scanty interstitial tissue and the alveolar lining cells (kind I or membranous kind occupying virtually a lobe known as pneumatocele. These cysts may pneumocytes challenge into the alveoli and are lined by include air or may get contaminated and become abscesses. The pores of Kohn are the websites of alveolar connections Intralobar sequestration is the sequestered bronchobetween the adjoining alveoli and allow the passage of micro organism pulmonary mass within the pleural covering of the affected and exudate. The main functions of lungs is oxygenation Extralobar sequestration is the sequestered mass of lung of the blood and removing of carbon dioxide. The respiratory tissue lying exterior the pleural investing layer such as within the tract is particularly exposed to an infection as well as to the base of left lung or beneath the diaphragm. The extralobar hazards of inhalation of pollutants from the inhaled air and sequestration is predominantly seen in infants and children cigarette smoke. There exists a natural mechanism of filtering and is usually related to other congenital malformations. The manufacturing of surfactant is normally elevated related morphology, and hence are discussed collectively beneath. The mechanism of acute harm by etiologic sudden and extreme respiratory distress, tachypnoea, brokers listed above depends upon the imbalance between protachycardia, cyanosis and extreme hypoxaemia. Infants born to diabetic mothers launch merchandise which cause active tissue harm. Delivery by caesarean section proteases, platelet activating issue, oxidants and 4. Shock as a result of sepsis, trauma, burns congestion, fibrin deposition and formation of hyaline 2. There is presence of collapsed alveoli (atelectasis) alterfactors listed above, and the final pathologic consequence of nating with dilated alveoli. Necrosis of alveolar epithelial cells and formation of how it occurs is different within the neonates than in adults. The membrane is essentially composed of fibrin outlined beneath: admixed with cell debris derived from necrotic alveolar cells. Interstitial and intra-alveolar oedema, congestion and formation of hyaline membrane i.

The protein product of S-gene plus activity could be achieved by boiling for 1 minute purchase ketoconazole cream 15gm without prescription bacteria weight loss, by ultraviolet radiation buy ketoconazole cream 15gm with mastercard antibiotic names, or by contact with formaldehyde and chlorine. The virus is current in the liver cells, bile, stool and blood during the incubation interval and in pre-icteric phase but viral shedding diminishes after the onset of jaundice. An immunologic basis is suspected but the proof in help is indirect in the type of immunologic markers but not direct demonstration of the etiologic agent in the affected hepatocytes. HbxAg has a role in transactivation the transcription of both viral and cellular genes. In these people it to fulminant hepatitis but fulminant hepatitis is extra doubtless persists for life providing safety in opposition to reinfection with in such simultaneous delta an infection. Its persistence look of severe and fulminant acute attacks, beyond 10 weeks is indicative of growth of chronic liver development of service stage to chronic delta hepatitis or disease and service state. About are Southern Europe, Middle-East, South India and elements of 1-2% of volunteer blood donors and as much as 5% of skilled Africa. Hepatitis C an infection is acquired by blood transfusions, blood products, haemodialysis, parenteral drug abuse and Figure 21. Carrier State Hepatitis E is an enterically-transmitted virus, beforehand An asymptomatic particular person with out manifest disease, labelled as epidemic or enterically transmitted variant of nonharbouring an infection with hepatotropic virus and able to A non-B hepatitis. There could be 2 types of aged people, primarily seen in India, other Asian carriers: nations, Africa and central America. The predominant histologic changes are: variable diploma of necrosis of hepatocytes, most marked in zone three (centrilobular); and mononuclear cellular infiltrate in the lobule. Mild diploma of liver cell necrosis is seen as ballooning degeneration whereas acidophilic Councilman our bodies (inbox) are indicative of extra severe liver cell damage. Icteric phase: the prodromal interval is heralded by the may not present changes on liver biopsy. The Asymptomatic carriers with chronic disease may present diagnosis is based on deranged liver operate tests (e. Post-icteric phase: the icteric phase lasting for about 1 raised serum transaminases or by detection of the presence to 4 weeks is usually followed by clinical and biochemical of antibodies but are in any other case asymptomatic. Acute Hepatitis acute hepatitis may develop severe type of the disease the commonest consequence of all hepatotropic viruses (fulminant hepatitis); and 5-10% of circumstances progress on to is acute inflammatory involvement of the whole liver. Grossly, the liver is Clinically, acute hepatitis is categorised into 4 phases: barely enlarged, gentle and greenish. The final named gives rise to i) Mildly injured hepatocytes appear swollen with autoimmune or lupoid hepatitis which is characterised by granular cytoplasm which tends to condense around the optimistic serum autoantibodies (e. Bridging necrosis is characterised by bands of the vulnerability of a patient of viral hepatitis to develop necrosis linking portal tracts to central hepatic veins, one chronic hepatitis are: impaired immunity and extremes of age central hepatic vein to another, or a portal tract to another at which the an infection is first contracted. Inflammatory infiltrate: There is infiltration by hepatitis activity score (described beneath. The frequency and mononuclear inflammatory cells, usually in the portal severity with which hepatotropic viruses trigger chronic tracts, but may permeate into the lobules. It is usually not possible to distinguish are as beneath: histologically between viral hepatitis of various etiologies, i) Necrosed hepatocytes on the limiting plate in but the next morphologic features may assist in giving periportal zone. Chronic Hepatitis characterised by variable diploma of changes in the portal tract. Chronic hepatitis is defined as persevering with or relapsing i) Inflammatory cell infiltration by lymphocytes, plasma hepatic disease for more than 6 months with signs alongside cells and macrophages (triaditis. Majority of circumstances of chronic iii) Additionally, chronic hepatitis C may present lymphoid hepatitis are the result of an infection with hepatotropic aggregates or follicles with reactive germinal centre and viruses�hepatitis B, hepatitis C and combined hepatitis B 612 Figure 21. Diagrammatic representation of pathologic changes in chronic hepatitis (B) contrasted with regular morphology (A. Photomicrograph on right (C) reveals stellate-shaped portal triad, with extension of fibrous spurs into lobules. The portal tract is expanded as a result of increased lymphomononuclear inflammatory cells that are seen to breach the limiting plate. Necroinflammatory activity: vii) Cases of chronic hepatitis B present scattered groundPeriportal necrosis i. The onset of fibrosis in chronic Intralobular necrosis, focal or confluent (ranging from score hepatitis from the world of interface hepatitis and bridging zero as none to attain 4 for >10 foci for focal necrosis, and necrosis is a function of irreversible harm. Extent and depth of portal irritation (ranging from grade necrosis by which the liver failure is speedy and fulminant 613 zero as no irritation to grade 4 having marked portal occurring in 2-three weeks. Fulminant hepatitis of either of the two varieties can happen from viral and non-viral etiologies: B. In addition, hepatitis are quite variable ranging from delicate disease to fullherpesvirus can also trigger severe viral hepatitis. Non-viral causes embrace acute hepatitis as a result of drug i) Mild chronic hepatitis reveals solely slight but persistent toxicity (e. The sufferers current with features of hepatic failure with hepatic encephalopathy (page 602. Grossly, the liver is small iv) Systemic features of circulating immune complexes due and shrunken, often weighing 500-700 gm. The sectioned floor reveals diffuse complex vasculitis, glomerulonephritis and cryoglobulior random involvement of hepatic lobes. Fulminant Hepatitis Regeneration in submassive necrosis is extra orderly and (Submassive to Massive Necrosis) may end in restoration of regular architecture. As a results of loss of hepatic parenchyma, all that Two patterns are recognised�submassive necrosis having a is left is the collapsed and condensed reticulin framework much less speedy course extending as much as three months; and big and portal tracts with proliferated bile ductules plugged Figure 21.

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The fundamental distinction between Psorinum and Arsenic is that in Psorinum cheap ketoconazole cream 15gm without a prescription how do antibiotics for acne work, the discharges have a robust dangerous scent discount ketoconazole cream 15 gm on line treatment for uti of dogs. If an offensive scent comes from the mouth, armpits and ft, with none apparent underlying disease, for this downside Rhus Glabra in potency 6X could be very efficient. If the Psoriasis is more distinguished on the palms of the hands and the soles of the ft, for that Hydrocotyle 385 additionally, Hydrocotyle ought to be used. If tasted somewhat carelessly, it could possibly cause severe laryngeal spasm (constriction of the throat), inflicting suffocation and quick dying. Since the amount of Hydrocyanic Acid found within the bitter almonds could be very little, the consumption of almonds might not even cause colic within the abdomen or spasm of the larynx, although some risk nonetheless remains. Most physicians have restricted using Hydrocyanic Acid to severe abdominal distension and severe colic, although it is extremely useful within the remedy of bronchial asthma, epilepsy and whooping cough. All these three circumstances could also be associated with constriction of the throat muscular tissues. If a affected person of bronchial asthma or whooping cough becomes unconscious because of the constriction of the higher respiratory passage, the affected person may be saved from serious potential problems with the quick use of Hydrocyanic Acid, otherwise because of the constriction of the air passages, the oxygen supply to the brain would be depleted, which sometimes results in everlasting brain harm. When given along with Hydrophobinum 200, it happens to work a lot better than the inhalers used within the allopathic system of medication. It is extremely useful within the remedy of hysterical matches and epileptic seizures in girls. Hydrocyanic Acid 387 Some sufferers develop cramps within the abdomen after taking meals and others before taking the meals. Hydrocyanic Acid is indicated in those sufferers who feel a colicky sort of pain on an empty stomach, or a marked weakness and sinking sensation over the stomach, or expertise a rumbling sound within the abdomen after taking meals. It is a very important anticonvulsive medicine, especially in treating the laryngeal spasm (constriction of the throat. The affected person goes into severe convulsions at the sight of shiny mild, shining objects or upon seeing water. The saliva of the affected person is like sticky threads as in Coccus Cacti and Kali Bichrome. Due to excessive hypersensitivity, the sound or even the thought of working water can irritate the symptoms. It profoundly impacts the mind, especially in relations to the sexual functioning. Sometimes, a affected person develops the symptoms of Hyoscyamus in the course of the typhoid fever. It is a typical symptom of Hyoscyamus, which causes inflammation of the sexual organs affecting the mind. The affected person talks in an absurd way that he would by no means do within the state of regular health. The affected person becomes very weak and his head slips and sags from the pillow because of the Hyoscyamus 391 weakness. He believes that somebody is standing next to him, and he starts conversing with him. If a affected person is afraid of taking the treatment via suspicion of being poisoned, a drop or two mixed in a large amount of water could also be administered. One of the ideas imprinted within the mind of the affected person is that he can by no means be forgiven for his sins. In truth, in his personal thoughts, he believes himself to have dedicated homicide or another heinous crime, though in actuality, he by no means dedicated such a crime. One symptom of Hyoscyamus, which resembles Glonoine and Lachesis, is that the affected person feels lost while on a journey. Kent, Belladonna, Hyoscyamus, Cantharis and Hydrophobinum are all wonderful medicines to treat this condition. In my expertise, I even have found Stramonium and Hydrocyanic Acid able to completely uprooting this condition. They are very efficient against the constriction of the throat growing on swallowing water. In the olden days, some docs used to treat the sufferers Hyoscyamus 392 with Stramonium. The sufferers who develop symptoms of Rabies ought to be handled with Stramonium and Hydrophobinum combined. These cures ought to first be given daily for a number of days, then twice every week for a number of months and then steadily tapered off. This will, by the grace of God, stop against the onset of Rabies and obviate any further dangers eternally. If the eyesight becomes weak because of the dysfunction of the nerves, then Hyoscyamus will be of good use. Sometimes, during a high fever, the affected person goes into a severe convulsion with the locking of the jaw. The tongue becomes insensitive and liable to be cut between the enamel while consuming meals. If all these symptoms are due to stomach upset, then Pulsatilla and Carbo Veg can be used. The stools (as in typhoid) are granular in appearance or could also be watery and mixed with blood. Another troubling downside is that the affected person has no management over passing urine or opening the bowels (urinary and faecal incontinence.

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The condition of alternating warmth and chilly changes frequently buy ketoconazole cream bacteria klebsiella infections, displaying that the fever is submerging deep down into the body order ketoconazole cream from india antibiotic during pregnancy. When the fever penetrates the spine or transfers itself in direction of the brain, the body turns into chilly. Nonetheless, the body retains on defending itself against the sickness and tries to expel it. In menopause, elderly girls undergo many numerous signs like scorching flushes on the face. Ladies who obtain reduction from using Camphor feel very chilly when uncovered but sweat profusely after they have their garments on. The headache behind the top and the lower a part of the neck, associated with a throbbing sensation, can presumably be handled with Camphor. Camphor could be very well-known for the remedy of convulsions leading to the lockjaw. Many a time, ladies undergo these sorts of signs as a result of intense warmth or marked inner weak spot. Low efficiency Camphor happens to be very effective by its mere utility to the lips. A characteristic function of Camphor is that in the state of convulsion and related lockjaw, the lips and tongue become blue. In Camphor, the tendency in direction of vomiting is extra frequent than the frequency of stools. The stools are frequent but scanty and are associated with marked weak spot of the body and cramps. For repeated loose motions, vomiting and cramping on the calves, Veratrum Album is the top most treatment. The body feeling chilly happens in each Camphor as well as Veratrum Album, but in Veratrum Album chilly sweats are visible solely on the forehead. When other ordinary treatments show ineffective, the chronic obstinate type of constipation can be relieved after a couple of doses of Veratrum Album. When the muscles of the urinary bladder become weak and one must strain the belly muscles to move urine, Camphor is a very useful treatment in this condition. Camphor could be very clearly useful for the remedy of this paralytic condition of the bladder. Whenever such Camphora 197 contrasting signs are noticed, Camphor should be stored in thoughts so that it might be used as wanted. Both these treatments assist in relieving the fixed tendency of contracting such illnesses. The thick sputum (expectoration) tends to be caught in the air passages leading to a feeling of suffocation and problem in respiration. The deep inspiration (inhaling) provokes coughing and the center starts beating quick. Another peculiar symptom of Camphor is that the ache feels considerably relieved by concentrating on the location of ache. After a significant surgery when the body turns into very chilly and the blood pressure falls profoundly, besides Carbo Veg a couple of doses of Camphor will offer immediate reduction. In the state of being unconscious, the Camphor patient reveals dilatation of the pupils of the eyes, which become fastened in one place. On exposure to chilly, the patient develops diarrhoea, the stools being darkish in color inflicting dehydration and profound weak spot. Adjuvant: Cantharis Antidotes: Phosphorus, Opium Potency: as much as 30 Camphora 198 Cannabis Indica Cannabis Indica is the other title for the cannabis grown in the Indian subcontinent. Time just about comes to a standstill for them and recent events look historical to them. The signs of Cannabis Indica in homoeopathy are described because the patient floating in the ambiance. In a normal person, this state of inertia would make him feel significantly oppressed. The patient of Cannabis Indica does very little but thinks he has worked for a lot of hours. He feels bodily agitated throughout, resulting in the shaking of the palms and toes. The signs of Cannabis very much resemble those of Opium, which is the best remedy for Catalepsy. The coexistence of laughing and crying like the sun and the shade, associated with a state of euphoria as an alternative of insanity, is the symptom of Cannabis Indica. Due to the intoxicating impact of Cannabis and damage to the brain, the patient could be very afraid of death. Cannabis in homoeopathic type shall be discovered useful in treating those people who exhibit these signs, yet are short of being intoxicated. The homoeopaths have accomplished the proving and have come to the conclusion that the thoughts of a Cannabis patient is bombarded with thoughts, and owing to his lack of ability to specific them, his speech is rather disjointed. He begins to speak regular after which abruptly turns into lost because of a new thought popping up. The Cannabis patient experiences a throbbing kind of headache associated with pounding of the center. It is an established incontrovertible fact that the thoughts of one person could also be transferred to a different individuals thoughts (telepathy. Some thoughts from a distant thoughts could also be detected through the so-known as sixth sense. If an individual reveals this sort of symptom as a result of any psychological dysfunction, Cannabis should be utilised.

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Calibration may be checked day by day by using the control cuvette supplied with the photometer order ketoconazole cream 15 gm free shipping infection control risk assessment. Preparation of a calibration graph to be used with a filter colorimeter discount ketoconazole cream 15 gm with mastercard antimicrobial mouthwash brands, 158 Hematology requires the use of a secondary blood normal, i. The absorbance of the solution is measured as oxyhemoglobin in a filter colorimeter utilizing a yellow-green filter or at wavelength 540nm. Preparation of calibration graph for HbO2 technique A series of dilutions are ready form a complete blood or normal hemolysate of known hemoglobin worth, 159 Hematology preferable between one hundred forty-160g/l. Prepare a 1 in 201 dilution of the usual blood or hemolysate within the ammonia water diluting fluid as follows: � Dispense 20ml of diluting fluid into a screw cap container. Tube 1: Hb worth of normal x four/5 = Hb g/l one hundred sixty Hematology Tube 2: Hb worth of normal x 3/5 = Hb g/l Tube 3: Hb worth of normal x 2/5 = Hb g/l Tube four: Hb worth of normal x 1/5 = Hb g/l Tube 5: Hb worth of normal = Hb g/l (no calculation required) 6 Take a sheet of graph paper and plot the absorbance of each normal (vertical axis) in opposition to its concentration in g/l (horizontal axis. Measure fastidiously 20�l of capillary or well-blended venous blood and dispense into 3. Using the desk ready from the calibration graph, learn off the sufferers hemoglobin worth. Disadvantage � Certain forms of Hb are resistant to alkali denaturation, in particular Hb-F and Hb Bart. The 162 Hematology sample is then cooled quickly in chilly water and when cool matched in opposition to the usual in a colour matched in opposition to the usual in a colorimeter at 540nm. Standard A mixture of chromium potassium sulphate, cobaltous sulphate and potassium dichromate in aqueous resolution. Most of the problems related to the Sahli technique are because of the instability of acid hematin, fading of the colour glass normal and problem in matching it to the acid hematin resolution. Principle 163 Hematology Hemoglobin in a sample of blood is transformed to a brown coloured acid hematin by therapy with zero. Materials Sahli hemoglobinometer Sahli pipette Stirring glass rod Dropping pipette Absorbent cotton zero. Fill the graduated tube to the '20' mark of the purple graduation or to the 3g/dl mark of the yellow graduation with zero. Blow the blood from the pipette into the graduated pipette into the graduated tube of the acid resolution. Compare the colour of the tube containing diluted blood with the colour of the reference tube. If the colour of the diluted sample is darker than that of the reference, continue to dilute by including zero. Depending on the kind of hemoglobinometer, this provides the hemoglobin concentration either in g/dl or as a proportion of 'regular'. A new low-value hemoglobin colour scale has been developed for diagnosing anemia which is dependable to within 10 g/l (l g/dl. It consists of a set of printed colour shades representing hemoglobin levels between four and 14 g/dl. The colour of a drop of blood collected onto a specific kind of absorbent paper is compared to that on the chart. Validation research in blood transfusion centers have proven the dimensions to be extra dependable and simpler to make use of than the copper sulphate technique in donor selection checks. Copper Sulphate Densitometery it is a qualitative technique primarily based on the capability of a standard resolution of copper sulphate to trigger the suspension or sinking of a drop of a sample of blood as a measure of particular gravity of the latter and similar to its hemoglobin concentration. The technique is routinely utilized in some blood banking laboratories within the screening of blood donors for the presence of anemia. Normal hemoglobin reference range: Children at birth one hundred thirty five-195 g/l kids 2 y � 5 y a hundred and ten-one hundred forty g/l Children 6 y � 12 y one hundred fifteen-a hundred and fifty five g/l Adult men one hundred thirty-a hundred and eighty g/l Adult women one hundred twenty-one hundred fifty g/l Pregnant women a hundred and ten-138 g/l 167 Hematology Review Questions 1. What are the two most commonly applied colour comparability methods for measurement of hemoglobin in a sample of bloodfi How do you check the linearity of the spectrophotometric technique of hemoglobin quantitation within the laboratoryfi It is likely one of the easiest, most correct and most precious of all hematological investigations. There are two methods of determination: microhematocrit technique and macrohematocrit (Wintrobe) technique. Microhematocrit technique Materials required � Capillary tubes these must be plain or heparinized capillaries, measuring 75mm in size with an inner diameter of 1mm and wall thickness of zero. The plain ones are used for 171 Hematology anticoagulated venous blood while the heparinized ones (inside coated with 2 I. Capillaries are finest sealed utilizing a plastic sealant, modeling clay, or plasticine. Test technique 1 Allow the blood to enter the tube by capillarity (if anticoagulated venous blood, adequate mixing is 173 Hematology necessary) leaving no less than 15mm unfilled (or fill 3/4th of the capillary tube. It is increased in hypochromic anemia, macrocytic anemia, sickle cell anemia, spherocytosis and thalassemia. Advantages of the Microhematocrit Method � It permits greater centrifugation speeds with consequent shorter centrifugation times and superior packing. A observe ought to be made on the sufferers report if an abnormal plasma or buffy coat is seen as this is usually an important clue for the clinician. If the plasma is pink-purple this indicates a hemolyzed sample (less generally hemoglobinemia.

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References:

  • https://deepblue.lib.umich.edu/bitstream/handle/2027.42/86353/ejra_1.pdf?sequence
  • http://ssu.ac.ir/cms/fileadmin/user_upload/Mtahghighat/taghzie_imani/book/Biochemistry%20For%20Dummies.pdf
  • http://brainimaging.waisman.wisc.edu/~lutz/VarelaHommage_Biological_research_2003.pdf
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